Biochemical and ultrastuctural alterations due to repetitive ethanol administration on the rat ventral prostate

DÍAZ GÓMEZ M.I., RODRIGUEZ DE CASTRO C., FANELLI S.L., DELGADO A.M.A.M COSTANTINI M.H., CASTRO G.D. Y CASTRO J.A.

Iguazú, Misiones

Congreso; IL Annual Meeting Sociedad Argentina de Investigación en bioquímica y Biología Molecular (SAIB); 2004

Sociedad Argentina de Investigación en bioquímica y Biología Molecular (SAIB)

In this work we report that rat prostate mitochondria (MIT)are able to bioactivate EtOH to AC and 1HEt.The enzymatic process leading to AC requires oxygen but not NADH or NADPH,is fully inhibited by 1mM diethyldithiocarbamate and is partially susceptible to 1mM deferoxamine.Incubation mixtures containing either microsomes or cytosol or mitochondria and (if necessary) cofactors plus EtOH lead to covalent binding of the reactive metabolites formed to their NaBH4-reduced proteins from the three fractions.Sprague-Dawley rats were fed with a nutritionally adequate liquid diet containing alcohol,for 28 days.This resulted in ultrastructural alterations in the epithelial cells,consisting of marked condensation of chromatin around their perinuclear membrane and of moderated dilatation of their endoplasmic reticulum.We also observed that this repetitive EtOH administration was accompanied of slightly increased peroxidability of prostatic lipids as detectable by the t- butylhydroperoxide promoted chemiluminscence emission test.Rat ventral prostate has several metabolic pathways for EtOH bioactivation to AC and 1HEt,some of which remain to be fully characterized.The reactive metabolites formed covalent bind to cellular components and are involved in the cell injury observed. Supported by CONICET (PIP02323) and UNSAM (PIDA UF013)