Ethanol toxicity on rat prostate and its role in prostate cancer related to alcohol drinking

DÍAZ GÓMEZ M; RODRIGUEZ DE CASTRO C; FANELLI SL; QUINTANS LN,; COSTANTINI MH; CASTRO JA; CASTRO GD.

Rosario, Santa Fe

Congreso; XLII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2006

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

The correlation between alcohol (EtOH) consumption and prostate cancer is controversial. It would be important to know whether prostate tissue leads to similar alterations to those attributed for EtOH promoted liver injury or cancer. These studies were performed in Sprague Dawley male rats fed with a nutritionally adequate liquid diet containing EtOH for 28 days and compared against adequately pair fed controls. Prostate microsomal fractions showed a CYP2E1-mediated EtOH metabolism and that activity was induced by repetitive EtOH drinking. Cytosolic activation of AC led to acetyl radical, as detected by spin trapping and GC-MS. Low activities of ADh and AlDh were observed in prostate tissue and AC accumulation occurred after EtOH administration. An increased oxidability of lipids was detected by chemiluminiscence emission and by increased levels of lipid hydroperoxides. Alterations in epithelial cells involved condensation of chromatin, dilatation of ER and some cells undergoing apoptosis. In summary, alcohol drinking leads to the formation of mutagenic AC and to tumor-promoting oxidative stress. However, it exerts direct and undirect proapoptotic effects in the prostate epithelial cells. The balance between both actions might explain, at least in part, many controversial results observed in epidemiological studies. Supported by CONICET (PIP 02323) and UNSAM (PIDA UF013).