RODRIGUES CAPITULO Alberto
Differential response between histological and biochemical biomarkers in the apple snail Pomacea canaliculata (Gasteropoda: Amullariidae) exposed to cypermethrin
ARRIGHETTI, F.; AMBROSIO, E.; ASTIZ, M.; CAPÍTULO RODRIGUES, A. ; LAVARIAS, S.
ELSEVIER SCIENCE BV
Año: 2018 vol. 194 p. 140 - 151
A B S T R A C TTo develop effective programs to monitor water quality is necessary to identify sensitive biomarkers in indicatorspecies. The aim of this study was to evaluate different biomarkers in the apple snail Pomacea canaliculataexposed to the insecticide Cypermethrin (CYP). Adult male and female snails were exposed to sublethal CYPconcentrations (10, 25 and 100 μg l−1) for 1, 4, 7 and 14 days. The recovery of the exposed snails was alsostudied by a post-exposure assay. The activities of the enzymes superoxide dismutase (SOD), catalase (CAT) andglutathione-S-transferase (GST), the levels of lipid peroxidation (LPO) and protein oxidation (PC) in digestivegland and gills were studied as biomarkers of exposure. Histopathological changes in target tissues were alsoevaluated. In digestive gland, CYP caused a significant increase in SOD, CAT and GST activities compared tocontrol (p < 0.05) as well as in LPO and PC levels (p < 0.05). However, such biochemical effects were neitherconcentration nor time dependent. Histopatological changes were observed in the exposed groups, such as anincrease in the number of basophilic cells, hemocytic infiltration and epithelia atrophy. Additionally, a positivecorrelation between the surface occupied by pigmented corpuscles and CYP concentrations was observed at allexposure periods. Gills showed greater sensitivity to oxidative damage than digestive gland. CYP caused an acutetoxic effect in LPO levels in this respiratory organ. The gill filament of exposed snails, exhibited a reduction orloss of cilia, vacuolization of the columnar cells and an increase in haemocyte content irrespective of the concentration.High concentrations of CYP caused disruptions in the columnar muscle fibers. In general, snails didnot show an improvement in their basal state during post-exposure treatment. Apparently, males and females donot have differential sensitivity to the pesticide. The results of this study suggest that histopathological changesare the most sensitive time- and dose-dependent biomarkers of toxicity induced by CYP in P. canaliculata.