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SMALDINI Paola Lorena

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Título:

Peptides from Amaranth controlled the NF-kB pathway activation on epithelial cells and suppressed intestinal inflammation

Autor/es:

SMALDINI PAOLA LORENA; QUEREDA, MICAELA; RIZZO, GASTON; ORSINI DELGADO, M LUCÍA; MORONTA, JULIAN; AÑON, MARIA CRISTINA; DOCENA, GUILLERMO H

Reunión:

Congreso; Reunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS); 2018

Resumen:

Biological, nutritional and health benefits of amaranth have beenhighlighted in the last years. Proteins of amaranth exert anti-hypertensive,anti-oxidant, anti-thrombotic and anti-proliferative effects. The aim of thisstudy was to analyze the anti-inflammatory effect of peptides from amaranth onNF-kB-intracellular pathway activation in intestinal epithelial cells, and in experimentalintestinal inflammation, such as colitis and food allergy.Colon cell lines (Caco-2 and Caco-luc) were cultured with flagellin andamaranth peptides. CCL20-expression was evaluated by qPCR and NF-κB modulationwas evaluation by light emission and qPCR, along with p65-nuclear traslocation.In vivo studies included the oral administration of a formulationcontaining the peptide during the allergic sensitization or the colitisinduction phase in Balb/c mice. Treatment efficacy was in vivo and in vitroevaluated.We found several peptides with anti-inflammatory capacity and weselected that with the highest ability to suppress cell activation (decrease inCCL20 and light emission p<0.05). Invivo studies showed, an amelioration of the clinical score (p<0.01) inthe food allergy mouse model, with inhibition of specific-IgE secretion(p<0.05) and negativitization of the cutaneous test (mean increase infootpad thickness control: 0.6mm vs peptide-treated: 0.3mm; p<0.05);intestinal nf-kb gene expression was reduced (fold change=3; p<0.01) along withup-regulation of tfg-b and foxp3. In the colitis mouse model, we found adecrease of the histologic score with a decrease expression and production ofpro-inflammatory cytokines (IL-1b, TNF and IFNg, p<0.05) and a decrease inthe myeloperoxidase activity in the peptide-treated group compared to control(p<0.05). NF-κB pathway was alsoabrogated in the gut.In conclusion, our findings indicated thatpeptides from amaranth endowed mucosal anti-inflammatory properties thatsuppressed the intestinal activation of NF-kB in Th1- and Th2-mediatedinflammation. These findings led us to propose that this peptide might be includedin the composition of a functional food.

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