INVESTIGADORES
SMALDINI Paola Lorena
congresos y reuniones científicas
Título:
ORAL IMMUNOTHERAPY USING SOY PROTEINS MODULATES THE ALLERGIC RESPONSE IN A MOUSE MODEL OF COW´S MILK ALLERGY
Autor/es:
SMALDINI, PAOLA; ORSINI DELGADO, M LUCÍA; CURCIARELLO, RENATA; CANDREVA, ANGELA MARIA; FOSSATI, CARLOS A; DOCENA, GUILLERMO H
Lugar:
Lima
Reunión:
Congreso; X Congreso de la Asociacion Latinoamericana de Inmunologia; 2012
Institución organizadora:
Asociación Latinoamericana de Inmunología
Resumen:
Food allergy is caused by a
breakdown in immunologic tolerance mechanisms. There is no approved therapy for
food allergies. In this work we aimed to induce oral tolerance to cow`s milk
proteins (CMP) using CMP and cross-reactive soy proteins (SP) in a food allergy
mouse model.
Balb/c
mice were gavaged for a week with CMP (CMP Tol) or with SP (PS Tol) prior to
sensitization by oral administration of CMP with choleric toxin. Finally, mice
were orally challenged with CMP. Immune response was evaluated with in vitro
(serum specific IgE, IgG1, IgG2a and IgA level; cytokine secretion and mRNA
expression, and induction of Foxp3 T cells in the intestinal mucosa and
splenocytes) and in vivo assays (clinical score and cutaneous tests).
We found a lower medium clinical
score in tolerized mice with CMP or PS after the oral challenge than in
sensitized mice, with a negative cutaneous test in tolerized mice. Specific IgE
[OD 1,57±0,2 vs 1,06±0.3 (SP Tol) or 0,52±0,1 (CMP Tol); p<0.05] and IgG1 [OD
2,16±0,3 vs 1,2±0.4 (SP Tol) or 0,11±0,02 (CMP Tol); p<0.01] were suppressed
in tolerized mice. IL-5 and IL-13 secretion was reduced in tolerized mice at the
protein and mRNA levels. We found inducible Tregs in mucosa.
In conclusion, oral immunotherapy based on cross-reactivity between CMP
and SP can be used to down-modulate the allergic specific immune response in CMP-sensitized
mice.