INVESTIGADORES
SMALDINI Paola Lorena
congresos y reuniones científicas
Título:
Characterization of a poly (allylamine hydrochloride) nanoparticle-based adjuvant that provides a rapid and broad mucosal immune activation
Autor/es:
DOCENA GUILLERMO H; RIZZO, GASTON; RODRIGO SANCHES; CHAVERO, CAMILA; BIANCHI, DAIANA; DUPUY EVANGELINA; APUZZO, EUGENIA; HERRERA, SANTIAGO; AGAZZI, MAXIMILIANO; AZZARONI, OMAR; OLIVEIRA, SERGIO; SMALDINI PAOLA L
Lugar:
Boston
Reunión:
Congreso; FOCIS; 2023
Resumen:
Different functionalnanoparticles (Np) can be designed based on different compositions, sizes,shape and surface properties for biomedical applications. This work aimed tocharacterize poly(allylamine) Np as a vehicle and adjuvant to be further usedin vaccines.Self-assembly Np were characterized using several antigen-presentingcells (APCs), epithelial cells and mice. Cell interaction and activation wasevaluated by fluorescence microscopy, flow cytometry and ELISA. Finally, Balb/cmice were systemically or mucosally immunized with Np-OVA and thepharmacokinetic and immunogenicity were evaluated.We found that APC internalized Np, which becameactivated, with a significantly increased expression of CD86 (p<0.05) andIL-1β (p<0.01). Using KO mice we demonstrated an NLRP3- and Caspase 1-dependentsecretion of IL-1β. In vivo tracking experiments showed that native Np native protectedOVA and reached the critical organs to promote immune activation. Then, a significantinduction of serum OVA-specific IgG and IgG2a, increased secretion of IFN-γ by spleencells and a high frequency of LT CD4+IFN-γ+ and LT CD8+IFN-γ+ cells were seen.Inconclusion, we found that APC internalized Np and activated the inflammasomepathway with IL-1β secretion. Remarkably, this nanoparticle exhibited adjuvantproperties for mucosal targeting to induce a Th1-dependent immunity that couldbe exploited in a vaccine.@font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:roman;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:swiss;mso-font-pitch:variable;mso-font-signature:-536859905 -1073732485 9 0 511 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin-top:0in;margin-right:0in;margin-bottom:10.0pt;margin-left:0in;line-height:115%;mso-pagination:widow-orphan;font-size:11.0pt;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-ansi-language:ES-AR;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-size:11.0pt;mso-ansi-font-size:11.0pt;mso-bidi-font-size:11.0pt;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-font-kerning:0pt;mso-ligatures:none;mso-ansi-language:ES-AR;}.MsoPapDefault{mso-style-type:export-only;margin-bottom:10.0pt;line-height:115%;}div.WordSection1{page:WordSection1;}