Memory reactivation triggers different patterns and an increment of brain HSC/HSP70 expression in the crab Chasmagnathus

KARINA ANDREA BARREIRO; JOSÉ CLEMENTE; LUIS DANIEL SUÁREZ; MARIANA FELD; ENRIQUE PORTIANSKY; ALEJANDRO DELORENZI

Córdoba Huerta Grande

Congreso; XXVII COGRESO ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN EN NEUROCIENCIAS & Reunión Satélite / Neurobiología del Comportamiento: ?Neuroetología y Neurobiología de la Memoria en el Cono Sur?; 2013

SOCIEDAD ARGENTINA DE INVESTIGACIÓN EN NEUROCIENCIAS

In the crabChasmagnathus memory model, animals associate the training context with avisual danger stimulus passing overhead. After a strong training (ST) crabs exhibitlong-term memory that can be expressed for 5 days, but after a weak training(WT) memory is not expressed after 8 hours. However, in both protocols a briefexposure to the training context (reminder) triggers memory reactivation and reconsolidation.In this study we used Heat Shock Protein HSC/HSP70 as a marker of changes inmetabolic activity. Although some of the neural substrates that support memoryin crabs have been shown in optic ganglia, brain areas activated by retrievalprocesses are still unexplored. We evaluated the changes in levels of HSC/HSP70protein expression in the brain after reminder sessions of ST and WT by westernblot. We found a significant increment of protein expression in WT crabs, and atendency in ST crabs. In addition, we evaluated changes in spatial patterns of HSC/HSP70expression in brain induced by memory reactivation. Inmunohistochemistry showedan increase tendency of expression in different neuropils of WT crabs, beingremarkable in the anteromedial neuropil. In further experiments we will testdifferent pharmacological treatments to demonstrate the dissociation betweenmechanisms mediating memory reactivation and those underling the behavioralexpression of memory.