Mapping Open Chromatin with Formaldehyde-Assisted Isolation of Regulatory Elements

NAMMO, T.; RODRÍGUEZ SEGUÍ, S. A.; FERRER, J.

Methods in Molecular Biology. Epigenetics Protocols

Humana Press

Año: 2011; p. 287 - 296

Noncoding regulatory genomic elements are central for cellular function, differentiation, and disease, but remain poorly characterized. FAIRE (formaldehyde-assisted isolation of regulatory elements) has emerged as a simple method to identify and analyze active regulatory sequences based on their decreased nucleosomal content. More recently FAIRE was combined with high-throughput sequencing (FAIRE-seq) to locate tissue-specific regulatory elements at a genome scale in purified human pancreatic islets. Here we describe the implementation of the FAIRE method in human pancreatic islet cells.