A subcellular atlas of AKT as a predictive tool of its physiological and pathological functions

ANTONELLA VILA; ALEJANDRO COLMAN-LERNER; MATÍAS BLAUSTEIN

Congreso; Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular - SAIB; 2022

Regulation of protein kinase AKT activity is associated with a diversity of processes, includingcell metabolism, growth, proliferation, differentiation and survival, as well as pathologicalprocesses such as viral infection and cancer development1.AKT is a therapeutic target for cancer treatment and it is known to be regulated throughnumerous posttranslational modifications (PTMs) as well as to be recruited to differentsubcellular compartments. However, little is known about how a cell determines whichsubstrates and functions AKT should regulate.Our hypothesis is that the profile of AKT PTMs can determine the subcellular localization ofAKT, and vice versa, thus establishing the subset of AKT target substrates and the set offunctions that AKT displays in response to each stimulus and each particular cellular context.The aim of this work is to develop an atlas of AKT subcellular localizations as a predictive toolof AKT physiological and pathological functions using bioinformatic and experimental tools.