STUDIES ON EISOSOMES DYNAMIC STRUCTURE IN Saccharomyces cerevisiae

CORREA, FRANCISCO; SALZMAN, VALENTINA; AGUILAR PS

Parana

Congreso; 54 Annual Meeting SAIB; 2018

SAIB

Eisosomes are protein and lipids complexes assembled onto the Plasmatic Membrane (PM) with sizes about 200nm long and 50nm wide. Thesestructures are distributed as elongated invaginations all over the Saccharomyces cerevisiae PM. The roles played by nanodomains in the cellularphysiology are known, however, the mechanism leading to their formation and maintenance is not completely comprehended. The evidencecollected by our group, and others too, allow us to postulate that eisosomes are shaped and maintained through the agency of scaffoldingconsisting in autoassembled proteins with BAR domains (bin-amphiphiysin-rvs) Pil1 and Lsp1. As the matter of fact, we know that Pil1 andSeg1 proteins are main scaffolding organizers while Lsp1 plays a secondary role. On the other hand, unpublished results hint that eisosomes´presence and shape are factors associated with the cell´s replicative lifespan. Considering this, we decided to study the eisosomes´ formation andmaintenance molecular dynamics on young cells while ageing. Our work on fluorescence Autocorrelation Function on eisosomes´ proteins inconjunction with design, fabrication and use of microfluidic devices will be key in order to study individual cells throughout its lifespan.