UNDERSTANDING EISOSOMES MEMBRANE DOMAINS´ ROLE IN AGING

VALENTINA SALZMAN; MARIBEL PATERNOSTE; PABLO S. AGUILAR

CABA

Congreso; Reunión Conjunta de Sociedades de Biociencias. 53 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2017

The plasma membrane (PM) of Saccharomyces cerevisiae, contains at least a dozen of different nanodomains. Eisosomes plasmamembrane nanodomains are reservoirs in of more than 25 proteins including transporters, signaling molecules, proteins reported to be involved incellular aging and proteins of unknown function. Eisosomes are structured in PM invaginations by scaffolds composed mainly by two cytoplasmicproteins Pil1 and Lsp1. In knockout strains of PIL1 domain´s organization is lost. Performing Chronological Longevity Survival (CLS) assays wefound that the Δpil1 mutant strain has an extended longevity phenotype. Loss of Pil1 increases lifespan additively with calorie restriction (CR) anddoes not influence glucose depletion. These results indicate that simulation of CR is not the underlying mechanism for Δpil1-dependet increase inlifespan. Understanding eisosomes´ role in aging will likely contribute to further describing S. cerevisiae aging process and domains function.