STUDIES ON EISOSOMES DYNAMIC STRUCTURE IN Saccharomyces cerevisiae AND THE USE OF MICROFLUIDIC CHIP TO MEASURE REPLICATIVE LIFESPAN

CORREA, FRANCISCO; MERCURI, M; BELLORA, F; MAZZEI, G; SALZMAN, V.; PASTORIZA, H; BELLINO, M; ESTRADA,L; AGUILAR, P

Congreso; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB); 2021

Eisosomes are fungal proteo-lipidic complexes assembled onto the plasma membrane (PM) with sizes about 200nm long and 50nm wide. In Saccharomyces cerevisiae, these structures are distributed as elongated invaginations all over the PM. Different roles played by these nanodomains are currently known, however, the mechanisms and protein dynamics leading to their formation and maintenance are not completely understood. The evidence collected by our group, and others too, allows us to postulate that eisosomes are shaped and maintained through self-assembling of BAR (bin- amphiphiysin- rvs) domaincontaining proteins Pil1 and Lsp1 (Olivera-Couto et. al 2011, doi: 10.1091/mbc.E10-12-1021). Unpublished results hint that eisosomes are factors associated with the cell’s replicative lifespan. To put this to the test, we developed a microfluidic device capable of trapping and isolating multiple individual S. cerevisiae cells throughout their whole lifespan. This device allows us to measure the replicative lifespan of different yeast strains and, with the use of confocal fluorescence microscopy, study protein dynamics changes as the cell ages throughout its lifespan.