PERSONAL DE APOYO
VACOTTO Marina
artículos
Título:
Hypoxia-induced Cell Death and Activation of Pro- and Anti-apoptotic Proteins in Developing Chick Optic Lobe
Autor/es:
MARINA VACOTTO; DANTE PAZ; SARA FISZER DE PLAZAS
Revista:
Neurochemistry Research
Editorial:
Springer
Referencias:
Año: 2006 vol. 31 p. 1003 - 1009
Resumen:
Exposure of the CNS to hypoxia is associated
with cell death. Our aim was to establish a temporal
correlation between cellular and molecular
alterations induced by an acute hypoxia evaluated at
different post-hypoxia (p-h) times and at two stages of
chick optic lobe development: embryonic days (ED) 12
and 18. TUNEL assays at ED12 disclosed a significant
increase (300%) in pyknotic cells at 6 h p-h, while at
ED18 no morphological changes were observed in
hypoxic versus controls. At ED12 there was a signifi-
cant increase (48%) in Bcl-2 levels at the end of the
hypoxic treatment, followed by a significant increase of
active caspase-9 (49%) and active caspase-3 (58%) at
30 and 60 min p-h, respectively, while at ED18 no
significant changes were observed. These findings
indicate that prenatal hypoxia produces an equilibrated
imbalance in both pro- and anti-apoptotic proteins
that culminates in a process of cell death, present
at earlier stages of development.