INVESTIGADORES
WEISSMANN Carina
artículos
Título:
IL-10 Inhibits Nitric Oxide Synthesis in Murine Uterus
Autor/es:
DIEGO OGANDO; MAXIMILIANO CELLA; MARÍA LAURA RIBEIRO; CARINA WEISSMANN; JULIETA AISEMBERG; ANA FRANCHI
Revista:
NEUROIMMUNOMODULATION.
Editorial:
Karger
Referencias:
Lugar: Basel; Año: 2004 vol. 11 p. 127 - 132
ISSN:
1021-7401
Resumen:
Objectives: Recent reports point to a role for the nitric
oxide/nitric oxide synthase (NO/NOS) system in implantation.
It has been suggested that inducible NOS expressed
at peri-implantation would lead to enhanced NO
production, which could promote the attachment of the
blastocyst. Short-term administration of NO donors during
the pre-implantation period reduced the pregnancy
rate in a dose-dependent manner. Thus, it is thought that
optimal levels of NO are critical for embryo implantation,
so regulation of NOS must be crucial. Taking this into
consideration, interleukin-10 (IL-10), synthesized and secreted
by the embryo, could be modulating NOS during
implantation. In this study we have investigated the in
vitro effect of IL-10 on NOS in the uterus. Methods: To
determine the effect of IL-10, slices of uterus from estrogenized
mice were pre-incubated for 60 min with different
concentrations of IL-10 and NOS activity was measured.Recent reports point to a role for the nitric
oxide/nitric oxide synthase (NO/NOS) system in implantation.
It has been suggested that inducible NOS expressed
at peri-implantation would lead to enhanced NO
production, which could promote the attachment of the
blastocyst. Short-term administration of NO donors during
the pre-implantation period reduced the pregnancy
rate in a dose-dependent manner. Thus, it is thought that
optimal levels of NO are critical for embryo implantation,
so regulation of NOS must be crucial. Taking this into
consideration, interleukin-10 (IL-10), synthesized and secreted
by the embryo, could be modulating NOS during
implantation. In this study we have investigated the in
vitro effect of IL-10 on NOS in the uterus. Methods: To
determine the effect of IL-10, slices of uterus from estrogenized
mice were pre-incubated for 60 min with different
concentrations of IL-10 and NOS activity was measured.Methods: To
determine the effect of IL-10, slices of uterus from estrogenized
mice were pre-incubated for 60 min with different
concentrations of IL-10 and NOS activity was measured.
Results: IL-10 (50 and 100 ng/ml in vitro) diminished
NOS activity. The in vivo administration of lipopolysaccharide
(LPS; 8 mg/kg) significantly increased the
conversion of arginine into citrulline. This effect was
abolished after 60 min of preincubation with IL-10
(100 ng/ml). The stimulatory effect of LPS and estrogen
on NOS activity is exerted on the Ca-independent isoform
and IL-10 in vitro abolished this increase. We
observed that the uterus of pregnant mice on day 5 of
gestation synthesized NO. This production was significantly
inhibited by preincubation with IL-10 (100 ng/ml).IL-10 (50 and 100 ng/ml in vitro) diminished
NOS activity. The in vivo administration of lipopolysaccharide
(LPS; 8 mg/kg) significantly increased the
conversion of arginine into citrulline. This effect was
abolished after 60 min of preincubation with IL-10
(100 ng/ml). The stimulatory effect of LPS and estrogen
on NOS activity is exerted on the Ca-independent isoform
and IL-10 in vitro abolished this increase. We
observed that the uterus of pregnant mice on day 5 of
gestation synthesized NO. This production was significantly
inhibited by preincubation with IL-10 (100 ng/ml).
Conclusions: This report demonstrates that IL-10 is capable
of inhibiting NO synthesis in estrogenized, LPStreated
and pregnant rat uterus.This report demonstrates that IL-10 is capable
of inhibiting NO synthesis in estrogenized, LPStreated
and pregnant rat uterus.