INVESTIGADORES
WEISSMANN Carina
artículos
Título:
Inverse and distinct modulation of tau-dependent neurodegeneration by presenilin 1 and amyloid-b in cultured cortical neurons: evidence that tau phosphorylation is the limiting factor in amyloid-b-induced cell death
Autor/es:
JULIA LESCHIK; ALFRED WELZEL; CARINA WEISSMANN; ANNE ECKERT; ROLAND BRANDT
Revista:
JOURNAL OF NEUROCHEMISTRY
Editorial:
Wiley Interscience
Referencias:
Año: 2007 vol. 101 p. 1303 - 1315
ISSN:
0022-3042
Resumen:
Alzheimers disease (AD) is characterized by massive neuron
loss in distinct brain regions, extracellular accumulations of
the amyloid precursor protein-fragment amyloid-b (Ab) and
intracellular tau fibrils containing hyperphosphorylated tau.
Experimental evidence suggests a relation between presenilin
(PS) mutations, Ab formation, and tau phosphorylation in
triggering cell death; however, how Ab and PS affect taudependent
degeneration is unknown. Using herpes simplex
virus 1-mediated gene-transfer of fluorescent-tagged tau
constructs in primary cortical neurons, we demonstrate that
tau expression exerts a neurotoxic effect that is increased with
a construct mimicking disease-like hyperphosphorylation
[pseudohyperphosphorylated (PHP) tau]. Live imaging
revealed that PHP tau expression is associated with increased
perikarya suggesting the development of a ballooned phenotype
as a specific feature of tau-mediated cell death.
Transgenic expression of PS1 suppressed tau-induced neurodegeneration.
In contrast, Ab amplified degeneration in the
presence of wt tau but not of PHP tau. The data indicate that
PS1 and Ab inversely modulate tau-dependent neurodegeneration
at distinct steps. They indicate that the mode by which
PHP tau causes neurotoxicity is downstream of Ab and that
tau phosphorylation is the limiting factor in Ab-induced cell
death. Suppression of tau expression or inhibition of tau
phosphorylation at disease-relevant sites may provide an
effective therapeutic strategy to prevent neurodegeneration in
Alzheimers disease.b (Ab) and
intracellular tau fibrils containing hyperphosphorylated tau.
Experimental evidence suggests a relation between presenilin
(PS) mutations, Ab formation, and tau phosphorylation in
triggering cell death; however, how Ab and PS affect taudependent
degeneration is unknown. Using herpes simplex
virus 1-mediated gene-transfer of fluorescent-tagged tau
constructs in primary cortical neurons, we demonstrate that
tau expression exerts a neurotoxic effect that is increased with
a construct mimicking disease-like hyperphosphorylation
[pseudohyperphosphorylated (PHP) tau]. Live imaging
revealed that PHP tau expression is associated with increased
perikarya suggesting the development of a ballooned phenotype
as a specific feature of tau-mediated cell death.
Transgenic expression of PS1 suppressed tau-induced neurodegeneration.
In contrast, Ab amplified degeneration in the
presence of wt tau but not of PHP tau. The data indicate that
PS1 and Ab inversely modulate tau-dependent neurodegeneration
at distinct steps. They indicate that the mode by which
PHP tau causes neurotoxicity is downstream of Ab and that
tau phosphorylation is the limiting factor in Ab-induced cell
death. Suppression of tau expression or inhibition of tau
phosphorylation at disease-relevant sites may provide an
effective therapeutic strategy to prevent neurodegeneration in
Alzheimers disease.b formation, and tau phosphorylation in
triggering cell death; however, how Ab and PS affect taudependent
degeneration is unknown. Using herpes simplex
virus 1-mediated gene-transfer of fluorescent-tagged tau
constructs in primary cortical neurons, we demonstrate that
tau expression exerts a neurotoxic effect that is increased with
a construct mimicking disease-like hyperphosphorylation
[pseudohyperphosphorylated (PHP) tau]. Live imaging
revealed that PHP tau expression is associated with increased
perikarya suggesting the development of a ballooned phenotype
as a specific feature of tau-mediated cell death.
Transgenic expression of PS1 suppressed tau-induced neurodegeneration.
In contrast, Ab amplified degeneration in the
presence of wt tau but not of PHP tau. The data indicate that
PS1 and Ab inversely modulate tau-dependent neurodegeneration
at distinct steps. They indicate that the mode by which
PHP tau causes neurotoxicity is downstream of Ab and that
tau phosphorylation is the limiting factor in Ab-induced cell
death. Suppression of tau expression or inhibition of tau
phosphorylation at disease-relevant sites may provide an
effective therapeutic strategy to prevent neurodegeneration in
Alzheimers disease.b and PS affect taudependent
degeneration is unknown. Using herpes simplex
virus 1-mediated gene-transfer of fluorescent-tagged tau
constructs in primary cortical neurons, we demonstrate that
tau expression exerts a neurotoxic effect that is increased with
a construct mimicking disease-like hyperphosphorylation
[pseudohyperphosphorylated (PHP) tau]. Live imaging
revealed that PHP tau expression is associated with increased
perikarya suggesting the development of a ballooned phenotype
as a specific feature of tau-mediated cell death.
Transgenic expression of PS1 suppressed tau-induced neurodegeneration.
In contrast, Ab amplified degeneration in the
presence of wt tau but not of PHP tau. The data indicate that
PS1 and Ab inversely modulate tau-dependent neurodegeneration
at distinct steps. They indicate that the mode by which
PHP tau causes neurotoxicity is downstream of Ab and that
tau phosphorylation is the limiting factor in Ab-induced cell
death. Suppression of tau expression or inhibition of tau
phosphorylation at disease-relevant sites may provide an
effective therapeutic strategy to prevent neurodegeneration in
Alzheimers disease.b amplified degeneration in the
presence of wt tau but not of PHP tau. The data indicate that
PS1 and Ab inversely modulate tau-dependent neurodegeneration
at distinct steps. They indicate that the mode by which
PHP tau causes neurotoxicity is downstream of Ab and that
tau phosphorylation is the limiting factor in Ab-induced cell
death. Suppression of tau expression or inhibition of tau
phosphorylation at disease-relevant sites may provide an
effective therapeutic strategy to prevent neurodegeneration in
Alzheimers disease.b inversely modulate tau-dependent neurodegeneration
at distinct steps. They indicate that the mode by which
PHP tau causes neurotoxicity is downstream of Ab and that
tau phosphorylation is the limiting factor in Ab-induced cell
death. Suppression of tau expression or inhibition of tau
phosphorylation at disease-relevant sites may provide an
effective therapeutic strategy to prevent neurodegeneration in
Alzheimers disease.b and that
tau phosphorylation is the limiting factor in Ab-induced cell
death. Suppression of tau expression or inhibition of tau
phosphorylation at disease-relevant sites may provide an
effective therapeutic strategy to prevent neurodegeneration in
Alzheimers disease.b-induced cell
death. Suppression of tau expression or inhibition of tau
phosphorylation at disease-relevant sites may provide an
effective therapeutic strategy to prevent neurodegeneration in
Alzheimers disease.
Keywords: Alzheimers disease, amyloid precursor protein/
Ab, herpes simplex virus, presenilin, tau, transgenic cortical
cultures.Alzheimers disease, amyloid precursor protein/
Ab, herpes simplex virus, presenilin, tau, transgenic cortical
cultures.b, herpes simplex virus, presenilin, tau, transgenic cortical
cultures.
J. Neurochem. (2007) 101, 13031315.(2007) 101, 13031315.