INVESTIGADORES
DE LA FUENTE Veronica
congresos y reuniones científicas
Título:
Mifepristone as a modulator of the multidrug resistant protein 1 on the blood brain barrier to increase the efficacy of doxorubicin on brain metastases
Autor/es:
RUBIN, AYELÉN; DE LA FUENTE V; CARABIAS P; TRONCOSO MF; LANARI C; ROJAS P
Lugar:
BUENOS AIRES
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
Institución organizadora:
SAIC, SAIB, SAI, SAA, SAB, SAB, SAFE, SAFIS, SAH, SAP
Resumen:
The presence of multidrug resistance efflux transporters, such asthe P-glycoprotein (P-gp) on the blood brain barrier (BBB), limits theefficacy of chemotherapeutic agents on brain tumors or metastases.The antiprogestin mifepristone (MFP) is known to inhibit P-gp activityon tumor cells. We hypothesize that MFP treatment improves theefficacy of Pegylated doxorubicin liposomes (doxo) on brain tumors.We used the murine mammary carcinoma C4-2-HI whose growth isnot inhibited by MFP treatment. Tumor cells (2x105) were injectedinto the brain of BALB/c-GFP mice using a stereotactic frame.Treatment with MFP (6 mg pellets, sc) and /or two weekly dosesof doxo (4,5 mg/kg, iv) was initiated 10 days after cell inoculation.We analyzed the results by fluorescence microscopy, measuring thetumor volume on brain slices after DAPI staining. Confirming thehypothesis, a significant decrease in tumor size was only observedin mice treated with MFP and doxo (p
