INTRAUTERINE EMBRYO POSITION IS ALTERED BY OBESITY PRIOR TO IMPLANTATION.

BAZZANO MARÍA VICTORIA; PAZ DANTE AGUSTÍN; ELIA EVELIN MARIEL

Puerto Varas

Congreso; VII SLIMP Reunión de la Sociedad Latinoamericana de Interacción Materno-Fetal y Placenta 2017; 2017

Sociedad Latinoamericana de Interacción Materno-Fetal y Placenta

Diverse signaling pathways, including adrenergic receptor beta 2 (β2AR), control intrauterine embryo positioning since implantation at a wrong place causes adverse effects on pregnancy outcome. However, the mechanisms underlying remain unclear and even less whether obesity affects them. Using cafeteria diet-induced obesity as animal model, we found that obesity causes aberrant uterine fetal distribution and macrosomia on gestational day (gd) 18.5. To elucidate whether this aberrant distribution is consequence of fetal reabsorptions or due to alterations in the foregoing embryo spacing, we analyzed the post implantation loss rates (PIL) and embryo positions just after (gd5.5) and before implantation (gd4.5). β2AR expression (qPCR and western blot) and localization (immunofluorescence) were analyzed on gd4.5. PIL was similar in control and obese rats; however aberrant uterine embryo distribution was detected on gd5.5 and gd4.5 in obese animals, indicating that obesity alters embryo positioning before implantation. On gd4.5, the total number of embryos (p