A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit

LUISA BERMUDEZ; URSULA URIAS; DANIELA MILSTEIN; LAURA KAMENETZKY; RAMON ASIS; ALISDAIR FERNIE,; MARIE VAN SLUYS; CARRARI, FERNANDO OSCAR; MAGDALENA ROSSI

Journal of Experimental Botany

OXFORD UNIV PRESS

Año: 2008 p. 2875 - 2890

In tomato, numerous wild-related species have been 15 demonstrated to be untapped sources of valuable genetic variability, including pathogen-resistance genes, nutritional, and industrial quality traits. From a collection of S. pennellii introgressed lines, 889 fruit metabolic loci (QMLs) and 326 yield-associated locidemonstrated to be untapped sources of valuable genetic variability, including pathogen-resistance genes, nutritional, and industrial quality traits. From a collection of S. pennellii introgressed lines, 889 fruit metabolic loci (QMLs) and 326 yield-associated lociS. pennellii introgressed lines, 889 fruit metabolic loci (QMLs) and 326 yield-associated loci 20 (YALs), distributed across the tomato genome, had been identified previously. By using a combination of molecular marker sequence analysis, PCR amplification and sequencing, analysis of allelic variation, and evaluation of co-response between gene expression(YALs), distributed across the tomato genome, had been identified previously. By using a combination of molecular marker sequence analysis, PCR amplification and sequencing, analysis of allelic variation, and evaluation of co-response between gene expression 25 and metabolite composition traits, the present report, provides a comprehensive list of candidate genes colocalizing with a subset of 106 QML and 20 YAL associated either with important agronomic or nutritional characteristics. This combined strategy allowedand metabolite composition traits, the present report, provides a comprehensive list of candidate genes colocalizing with a subset of 106 QML and 20 YAL associated either with important agronomic or nutritional characteristics. This combined strategy allowed 30 the identification and analysis of 127 candidate genes located in 16 regions of the tomato genome. Eightyfive genes were cloned and partially sequenced, totalling 45 816 and 45 787 bases from S. lycopersicumthe identification and analysis of 127 candidate genes located in 16 regions of the tomato genome. Eightyfive genes were cloned and partially sequenced, totalling 45 816 and 45 787 bases from S. lycopersicumS. lycopersicum and S. pennellii, respectively. Allelic variation at theS. pennellii, respectively. Allelic variation at the 35 amino acid level was confirmed for 37 of these candidates. Furthermore, out of the 127 gene-metabolite co-locations, some 56 were recovered following correlation of parallel transcript and metabolite profiling. Results obtained here represent the initial steps in the integration of genetic, genomic, and expressional 4040 patterns of genes co-localizing with chemical compositional traits of the tomato fruit.amino acid level was confirmed for 37 of these candidates. Furthermore, out of the 127 gene-metabolite co-locations, some 56 were recovered following correlation of parallel transcript and metabolite profiling. Results obtained here represent the initial steps in the integration of genetic, genomic, and expressional 4040 patterns of genes co-localizing with chemical compositional traits of the tomato fruit.