Zooming-in on a Quantitative Trait Nucleotide (QTN) for Tomato Yield using Wild Species Introgression Lines.

FRIDMANN, E; CARRARI, F; YONG-SHENG LIU; FERNIE AR; ZAMIR, D

SCIENCE

AMER ASSOC ADVANCEMENT SCIENCE

Año: 2004 p. 1786 - 1789

0036-8075

To explore natural biodiversity we developed and examined introgression lines (ILs) containing chromosome segments of wild species (Solanum pennellii) in the back- ground of the cultivated tomato (S. lycopersicum). We identified Brix9-2-5, which is a S. pennellii quantitative trait locus (QTL) that increases sugar yield of tomatoes and was mapped within a flower- and fruit-specific invertase (LIN5). QTL analysis representing five different tomato species delimited the functional polymorphism of Brix9-2-5 to an amino acid near the catalytic site of the invertase crystal, affecting enzyme kinetics and fruit sink strength. These results underline the power of diverse ILs for high-resolution perspectives on complex phenotypes.