NEURONAL DIFFERENTIATION OF MURINE NEUROBLASTOMA N2A CELLS IS DIFFERENTIALLY INDUCED BY SELECTIVE INHIBITION OF CLASS I OR CLASS IIA HDACS

JUNGE S; BERNARDI A; VILLALBA S; URBANO FJ; BISAGNO V

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

Sociedades SAIC SAB AAFE AACYTAL

Histone deacetylases (HDACs) are vital enzymes for regulating chromatin functions. Their primary role is to eliminate acetyl groups allowing histones to wrap the DNA more tightly. This study aims to investigate the in vitro application of selective inhibitors targeting class I/IIa HDACs. HDAC inhibitors (HDACi) are upcoming interesting targets due to their involvement in epigenetic/non-epigenetic regulation, and their potential as use as anti-cancer agents. All statistical differences were tested using ANOVAs. We used N2a cell cultures, a fast-growing mouse neuroblastoma cell line, capable of differentiating into neurons. They were cultured for 4 or 7 days in vitro (d.i.v.) using a 24-well plate in a DMEM with low serum (0.5%) condition and treated with HDACi MS275 (class I) and MC1568 (class IIa), at high or low concentrations. Equivalent DMSO concentrations were used as control. Whole cell patch-clamp recordings were performed to study neuron-like characteristics. Results showed a severe decrease in cell viability 4 d.i.v with MS275 (high), further corroborated by dapi staining. The same tendency was observed at 7 d.i.v (p