INVESTIGADORES
MUÑOZ Manuel Javier
artículos
Título:
Identification of the cellular targets of the transcription factor TCERG1 reveals a prevalent role in mRNA processing
Autor/es:
PEARSON JL, ROBINSON TJ, MUÑOZ MJ, KORNBLIHTT AR, GARCIA-BLANCO MA.
Revista:
JOURNAL OF BIOLOGICAL CHEMISTRY
Referencias:
Año: 2008 vol. 283 p. 7949 - 7961
ISSN:
0021-9258
Resumen:
The transcription factor TCERG1 (also known as CA150)
associates with RNA polymerase II holoenzyme and alters the elongation
efficiency of reporter transcripts. TCERG1 is also found as a component of
highly purified spliceosomes and has been implicated in splicing. To elucidate
the function of TCERG1, we used short interfering RNA-mediated knockdown
followed by en masse gene expression analysis to identify its cellular targets.
Analysis of data from HEK293 and HeLa cells identified high confidence targets
of TCERG1. We found that targets of TCERG1 were enriched in microRNA-binding
sites, suggesting the possibility of post-transcriptional regulation.
Consistently, reverse transcription-PCR analysis revealed that many of the
changes observed upon TCERG1 knockdown were because of differences in
alternative mRNA processing of the 3'-untranslated regions. Furthermore, a novel
computational approach, which can identify alternatively processed events from
conventional microarray data, showed that TCERG1 led to widespread alterations
in mRNA processing. These findings provide the strongest support to date for a
role of TCERG1 in mRNA processing and are consistent with proposals that TCERG1
couples transcription and processing.