ANALYSIS OF THE INTERACTION BETWEEN THE GLUCOCORTICOID RECEPTOR AND PROGESTINS

MARINI, MS; OGARA, MF; STORTZ M; LEVI V; PECCI A

Cordoba

Congreso; LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Glucocorticoid receptors (GR) and progestins receptors (PR) are members of the family of steroid receptors. The active PR is associated with cell proliferation and progression mammary tumors. GR activation promotes cell differentiation. Thus, the relative abundance of both receptors may modulate the proliferative response of the mammary epithelium. In view of these precedents, the objective of this work it was to test the ability of both receptors to be part of the same complex and study the combined action of these genes expression on shared targets. We used fluorescence correlation spectroscopy (FCS). It allows to obtain quantitative parameters related to the mobility of the molecules and their interaction with specific targets in living cells. From these analyzes it was observed that both receptors activated with their respective ligands within the cell nucleus are found in the same place and move simultaneously. We also noticed that the active GR could interfere with the time of the PR in a random region of the nucleus. We also found that both activated receptors could regulate the expression of specific genes involved in cell differentiation and proliferation. These results suggest that PR and GR could be part of the same protein complex and play an important role in the cellular response of the mammary epithelium.