INVESTIGADORES
FREUDENTHAL Ramiro A. M.
congresos y reuniones científicas
Título:
Chemical LTP induce changes in the acetylation state of synaptic protein and PSD-95 clustering in mice hippocampal neurons in culture
Autor/es:
SEBASTIAN RIVAS; MARÍA FLORENCIA ACUTAIN; VERÓNICA BÁEZ; RAMIRO FREUDENTHAL
Lugar:
Buenos Aires
Reunión:
Congreso; SAN 2020; 2020
Institución organizadora:
SAN
Resumen:
Protein acetylation affects synaptic plasticity and memory, but its effects on synaptic composition are poorly understood. Previous work of our group shows a memory facilitation effect of an inhibitor of the cytoplasmic histone deacetylase 6. The literature reports an increase in the clustering of PSD-95 after TSA treatment and also an augmented microtubule spine entry frequency after LTP induction in hippocampal neurons in culture (Catarino, 2013; Shatzle, 2018).We evaluate the changes induced by a high glycine LTP protocol (chLTP) in the PSD-95, synapsin and acetylated lysine clusters. The chLTP was applied to 18 DIV mice hippocampal neurons in culture and evaluated before treatment (ctrl), 0, 5, 15 and 45 minutes after it
