INVESTIGADORES
FREUDENTHAL Ramiro A. M.
artículos
Título:
Characterization of the beta amyloid precursor protein-like gene in the central nervous system of the crab Chasmagnathus. Expression during memory consolidation.
Autor/es:
MARIA SOL FUSTIÑANA; PABLO ARIEL; NOEL FEDERMAN; RAMIRO FREUDENTHAL; ARTURO ROMANO
Revista:
BMC NEUROSCIENCE
Editorial:
BIOMED CENTRAL LTD
Referencias:
Año: 2010 vol. 2010 p. 109 - 109
ISSN:
1471-2202
Resumen:
ABSTRACTBackground: Human β-amyloid, the main component in the neuritic plaques found inpatients with Alzheimers disease, is generated by cleavage of the β-amyloidprecursor protein. Beyond the role in pathology, members of this protein family aresynaptic proteins and have been associated with synaptogenesis, neuronal plasticityand memory, both in vertebrates and in invertebrates. Consolidation is necessary toconvert a short-term labile memory to a long-term and stable form. Duringconsolidation, gene expression and de novo protein synthesis are regulated in order toproduce key proteins for the maintenance of plastic changes produced during theacquisition of new information. Results: Here we partially cloned and sequenced thebeta-amyloid precursor protein like gene homologue in the crab Chasmagnathus(cappl), showing a 37% of identity with the fruit fly Drosophila melanogasterhomologue and 23% with Homo sapiens but with much higher degree of sequencesimilarity in certain regions. We observed a wide distribution of cappl mRNA in thenervous system as well as in muscle and gills. The protein localized in all tissuesanalyzed with the exception of muscle. Immunofluorescence revealed localization ofcAPPL in associative and sensory brain areas. We studied gene and proteinexpression during long-term memory consolidation using a well characterizedmemory model: the context-signal associative memory in this crab species. mRNAlevels varied at different time points during long-term memory consolidation andcorrelated with cAPPL protein levels, Conclusions: cAPPL mRNA and protein iswidely distributed in the central nervous system of the crab and the time course ofexpression suggests a role of cAPPL during long-term memory formation.
