INVESTIGADORES
SREBROW Anabella
artículos
Título:
Regulation of fibronectin alternative splicing by a basement membrane-like extracellular matrix.
Autor/es:
ANABELLA SREBROW; MATIAS BLAUSTEIN; ALBERTO R KORNBLIHTT
Revista:
FEBS LETTERS
Editorial:
Federation of European Biochemical Societies. Published by Elsevier Science B.V.
Referencias:
Año: 2002 vol. 514 p. 285 - 289
ISSN:
0014-5793
Resumen:
Hepatocytes are the source of plasma fibronectin
(FN) which lacks the alternatively spliced EDI segment,
distinctive of oncofetal FN. When hepatic or other epithelial
cells are cultured on plastic, EDI inclusion is triggered. Here we
report that EDI inclusion is inhibited when hepatic cells are
cultured on a basement membrane-like extracellular matrix
(ECM), demonstrating a new role for the ECM in the control of
gene expression. The effect is duplicated by collagen IV and
laminin but not by collagen I; is not observed with another
alternatively spliced FN exon (EDII); and correlates with a
decrease in cell proliferation, consistently with high EDI
inclusion levels observed in many physiological and pathological
proliferative processes.