INVESTIGADORES
SREBROW Anabella
artículos
Título:
Induction of the angiogenic phenotype by Hox D3.
Autor/es:
BOUDREAU N; ANDREWS C; SREBROW A; RAVANPAY A; CHERESH D
Revista:
JOURNAL OF CELL BIOLOGY
Editorial:
ROCKEFELLER UNIV PRESS
Referencias:
Año: 1997 vol. 139 p. 257 - 264
ISSN:
0021-9525
Resumen:
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Angiogenesis
is characterized by distinct phenotypic changes in vascular endothelial cells
(EC). Evidence is provided that the Hox D3 homeobox gene mediates conversion of
endothelium from the resting to the angiogenic/invasive state. Stimulation of
EC with basic fibroblast growth factor (bFGF) resulted in increased expression
of Hox D3, integrin alphavbeta3, and the urokinase plasminogen activator (uPA).
Hox D3 antisense blocked the ability of bFGF to induce uPA and integrin
alphavbeta3 expression, yet had no effect on EC cell proliferation or
bFGF-mediated cyclin D1 expression. Expression of Hox D3, in the absence of
bFGF, resulted in enhanced expression of integrin alphavbeta3 and uPA. In fact,
sustained expression of Hox D3 in vivo on the chick chorioallantoic membrane
retained EC in this invasive state and prevented vessel maturation leading to
vascular malformations and endotheliomas. Therefore, Hox D3 regulates EC gene
expression associated with the invasive stage of angiogenesis.
