First report of Cowpea mild mottle virus in chia (Salvia hispanica)

CELLI M G; PEROTTO M C; MERINO M C; NOME DOCAMPO, C.F.; FLORES C.; CONCI V. C

CROP PROTECTION

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2016

0261-2194

First report of Cowpea mild mottle virus in chía (Salvia hispanica) Celli, MG1, Perotto, MC1,2, Merino, MC2,Nome, CFD2, Flores, CR3, Conci, VC1,2,* 1Consejo Nacional deInvestigaciones Científicas y Técnicas ? CONICET, 2Inst. dePatología Vegetal (IPAVE) ? INTA, Con. 60 cuadras Km 5,5 (5119) Córdoba,Argentina, 3Estación Experimental Agropecuaria Yuto ? INTA. *E-mail:conci.vilma@inta.gob.ar AbstractChía (Salvia hispanica), an herbaceous plant of the Lamiaceae family, has displayed a growing popularity in the lastsyears due to its high concentrations of the health-beneficial Omega-3 fattyacid. The aim of this study was to characterize the viruses affecting thiscultivation. A total of forty chía plants showing leaf deformation,dwarfism or chlorosis were collected from production fields located inArgentina northeast. The samples were screened for the presence of Alfalfa mosaic virus (AMV), Cowpea mild mottle virus (CPMMV), Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Tospovirus group (I, II and III), Potyvirus and Begomovirus genus. Threeplants were positive for CPMMV. When CPMMV positive samples were analyzed byelectron microscopy, presence of feather-likeinclusions formed by presumed virions wereobserved. Moreover, ORF2 to ORF6 from the CPMMV viral genome was amplified inby RT-PCR. Viral coat-protein (CP ? ORF5) was analyzed against viral CPMMVisolates annotated in GenBank. The nucleotides identity was between 75.6% and99.0%, above given International Committee on Taxonomy of Viruses (ICTV)criteria for differentiation of Carlavirus species. The phylogenetic analysisrevealed that the isolates found in chía plants grouped with other isolatesfrom Brazil, Ghana and USA, but separate from those from India. In the presentwork we report, for the first time, the presence of Cowpea mild mottle virus in chía.