Liquid chromatography tandem-mass spectrometry (LC-MS/MS) and dried blood spot sampling applied to pharmacokinetics studies in animals: Correlation of classic and block design

BALDO, MATÍAS N; ANGELI, EMMANUEL; GAREIS, NATALIA C; HUNZICKER, GABRIEL A; MURGUÍA, MARCELO C; ORTEGA, HUGO H; HEIN, GUSTAVO J

LABORATORY ANIMALS

ROYAL SOC MEDICINE PRESS LTD

Año: 2017 vol. 52 p. 125 - 134

0023-6772

A relative bioavailability study (RBA) of two phenytoin (PHT) formulations was conducted in rabbits, in order to compare the results obtained from different matrices (plasma and blood from dried blood spot (DBS) sampling) and different experimental designs (classic and block). The method was developed by liquid chromatography tandem-mass spectrometry (LC-MS/MS) in plasma and blood samples. The different sample preparation techniques, plasma protein precipitation and DBS, were validated according to international requirements. The analytical method was validated with ranges 0.20-50.80 and 0.12-20.32 µg ml-1, r > 0.999 for plasma and blood, respectively. Accuracy and precision were within acceptance criteria for bioanalytical assay validation (< 15 for bias and CV% and