Effect of Parenteral Supplementation of Minerals and Vitamins on Oxidative Stress Biomarkers and Hepatic Fatty Acid Metabolism in Dairy Cows During the Transition Period

BARCAROLO, DAIANA; ANGELI, EMMANUEL; ETCHEVERS, LUCAS; RIBAS, LUCAS E.; MATILLER, VALENTINA; REY, FLORENCIA; ORTEGA, HUGO H.; HEIN, GUSTAVO J.

BIOLOGICAL TRACE ELEMENT RESEARCH

HUMANA PRESS INC

Año: 2024 vol. 202 p. 1582 - 1593

0163-4984

In the present work we aimed to study the effects ofparenteral vitamin and mineral supplementation on hepatic fatty acid metabolismas well as on the oxidative stress biomarkers in biological samples oftransition cows. The supplemented group (SG, n = 11) received a subcutaneous injection of 5 mL of vitamin Apalmitate 35 mg/mL, vitamin E acetate 50 mg/mL plus other injection of 5 mL ofcopper edetate 10 mg/mL, zinc edetate 40 mg/mL, manganese edetate 10 mg/mL andsodium selenite 5 mg/mL on days -60, -30 and 7 (± 3) relative to calving. Thecontrol group (CG, n = 11) receivedtwo subcutaneous injections of 5 mL of 9 mg/mL sodium chloride at the sametimes of the SG. Blood, urine and liver biopsies were sampled 21 (±3) daysbefore the expected calving date and 7 and 21 (±3) days after calving. Resultsrevealed that supplemented animals had higher glutation peroxidase (GSH-Px) activity,lower and higher concentration of 3-nitrotyrosine (3-NT) in the liver andplasma, respectively, higher expression of the mitochondrial beta-oxidationenzyme carnitine palmitoyltransferase 1 in the liver, and lower content of hepatictriacylglycerol, mirroring plasma liver function parameters. No differencesbetween groups were found in the superoxide dismutase activity, MDAconcentrations, the protein abundance of peroxisomal acyl-CoA oxidase 1,diacylglycerol O-acyltransferase 1 and peroxisome proliferator-activatedreceptor alpha. These results suggest that the vitamin and mineral supplementationprovided to dairy cows had a beneficial effect on GSH-Px activity, hepatic 3-NTconcentration and on the metabolic adaptation during the peripartum period.