Detection of 2-branched (1,3)-beta-D-glucan producing lactic acid bacteria by a qPCR assay and quantification of the polysaccharides by a competition ELISA method

PALOMA LÓPEZ; MARÍA LAURA WERNING,; IDOIA IBARBURU; PATRICIA ELIZAQUÍVEL; PILAR FERNÁNDEZ DE PALENCIA; ANA IRASTORZA; MARÍA TERESA DUEÑAS; ROSA AZNAR

Kosice

Conferencia; INTERNATIONAL SCIENTIFIC CONFERENCE PROBIOTICS AND PREBIOTICS; 2010

AbstractMost of the commercialized probiotics are limited to a few strains of Bifidobacteria, Lactobacilli and Streptococci, most of which produce exopolysaccharides (EPS). This fact, together with reports on immunomodulating ability as well as anticarcinogenic and cholesterol-lowering activities of EPS-producing lactic acid bacteria (LAB), suggest that the beneficial properties of these micro-organisms for human health may be due to the biological activities of these prebiotic biopolymers, whose producing bacteria are also frequently used to improve texture and taste of dairy products. LAB strains belonging to the Pediococcus, Lactobacillus and Oenococcus genera, isolated from cider and wine, produce a 2-substituted (1, 3)-b-D-glucan (b-D-glucan). Analysis of the rheological properties of the b-D-glucan synthesized by P. parvulus 2.6 showed that it has potential utility as a biothickener. In addition, the EPS confers to 2.6 strain increased adherence to Caco-2 cells as well as alteration of its immunomodulation of macrophages. Thus, methods for detection of b-D-glucan producing LAB as well as for quantitative detection of their EPS could be useful for the identification and evaluation of new potential probiotic strains