Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution

DALLARD BE; PUJATO S; BARAVALLE C; PEREYRA EAL; REY F; RENNA M.S; CALVINHO LF

RESEARCH IN VETERINARY SCIENCE

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2013 p. 462 - 470

0034-5288

The objective of this study was to evaluate the effects of a single intramammary infusion of Panax ginseng 25Panax ginseng 25 extract (GS) on insulin-like growth factors (IGF) in bovine mammary gland during early involution. Eight 2626 mammary quarters from six nonpregnant cows in late lactation were infused with 10 mL of ginseng 27mL of ginseng 27 extract solution (3 mg/mL), six quarters were treated with 10 mL of placebo (vehicle alone) and six quar- 28mg/mL), six quarters were treated with 10 mL of placebo (vehicle alone) and six quar- 28 ters were maintained as uninoculated controls. Milking was interrupted after infusion. Concentrations of 2929 IGF1 in mammary secretions were higher in GS-treated quarters than in placebo and uninoculated con- 3030 trol quarters at 24, 48 and 72 h post-treatment (p < 0.05). Treatment with GS did not affect mammary 31p < 0.05). Treatment with GS did not affect mammary 31 secretion of IGF2 (p = 0.942). At 7 d of post-lactational involution, a decrease of immunostained area 32p = 0.942). At 7 d of post-lactational involution, a decrease of immunostained area 32 and mRNA expression for IGF1 was observed in mammary tissue of GS-treated quarters compared with 3333 placebo-treated quarters and uninoculated controls (p < 0.05). The IGF2 immunostained area and mRNA 34p < 0.05). The IGF2 immunostained area and mRNA 34 expression for this growth factor were not affected by GS treatment (p = 0.216 and p = 0.785, respec- 35p = 0.216 and p = 0.785, respec- 35 tively). An increase in protein levels and mRNA expression in mammary tissue of IGFBP3, IGFBP4 and 3636 IGFBP5 was observed in GS-treated quarters compared with placebo-treated quarters and uninoculated 3737 controls (p < 0.05). These results provide evidence that intramammary inoculation of GS extract at cessa- 38p < 0.05). These results provide evidence that intramammary inoculation of GS extract at cessa- 38 tion of milking may promote early mammary involution through the inhibition of IGF1 local production 3939 and bioavailability.