Lectinhistoquímica en quistes foliculares inducidos en ratas.

SALVETTI, NR; BARBEITO, CG; GIMENO, EJ; ORTEGA, HH

La Plata, Buenos Aires. Agosto.

Congreso; IX Congreso de la Sociedad de Ciencias Morfológicas de La Plata - VI Jornadas de Educación.; 2007

The aim of this study was to detect changes in the lectinhistochemistry binding pattern in the wall of ovarian cysts.  Wistar rats were housed in permanent light rooms along 90 days. A control group was housed in a standard ilumination pattern, this animals were sacrificed in proestrus. Samples of the ovaries were processed to incubation with the followings byotinilated lectins: WGA, Con-A, DBA, SBA, PNA, RCA-1 and UEA-1. We analysed the granulosa of normal, atretic and cystic follicles; interstitial cells, zona pellucida, mesothelium and vessels, in both groups, and in corpora lutea in the controls. We found loss of PNA and SBA binding in granulosa of the cysts in relation to the growing follicles. The PNA, SBA and WGA binding dissapear in the interstitial cells of the ovaries with cyst follicles. Residues of zona pellucida found in the atretic follicles of the cystic ovaries haven´t the Con-A affinity characteristic of the growing follicles. In the granulosa, the lectin affinity was minor in the atretic follicles. The marcation staining in the endothelium is similar in both groups. In conclusion, there is a minor lectin binding, and consequently a minor expression of sacharides in the cystic ovaries. The sacharides lost could be: N acetyl-D galactosamine and D-galactose. These carbohydrates could be related with different physiology processes. The changes are added to other found in previous studies, as modifications in growth factors and cytoskeleton proteins.