Color Doppler, ultrasound, and pathologic features of spontaneous leydig cell carcinoma of testis in Balb/c Cmedc mice

SACCO SOFIA,; BELOTTI EDUARDO MATÍAS; BERTONA JUDITH; SALINAS FACUNDO; SALVETTI NATALIA RAQUEL; ORTEGA HUGO HÉCTOR

Washington DC

Congreso; ACVP and ASVCP Concurrent Annual Meeting; 2018

ACVP and ASVCP

Background: Carcinomas of the testis occur spontaneously in Balb/c mice. The colordoppler sonographic appearance of Leydig cell carcinoma (LCC) has not beendescribed in mice.Objective: To describe the ultrasound, color Doppler and pathologic features ofspontaneous LCC of the testis in Balb/c CMedC mice.Methods: Two, 11 and 12-month-old, respectively, Balb/c CMedC male mice with ahistory of aggression, anorexia, depression and ascites were submitted for diagnosticwork up. The testicles were enlarged, reddish and bluish; they were examined with acolor Doppler ultrasoundZ6Vet. Necropsies were performed and selected tissues werecollected for histopathology.Results: Ultrasound showed a large, multinodular, oval mass of heterogeneousechotexture in one testicle of each mouse. Color doppler revealed central andperipheral, intense hyper-vascularization. Grossly, the tumors were unilateral white toyellowish nodules (10-13 mm diameter) with multifocal cystic cavities and hemorrhagicareas. Histology showed a unencapsulated, multilobulated and densely-cellularneoplasm. This mass was composed of sheets of polyhedral cells with eosinophilic,granular or vacuolated cytoplasm. The cells were supported by a fine fibrovascularstroma. A central round nucleus with stippled chromatin and 1-3 magenta nucleoli waspresent. Marked anisocytosis and anisokaryosis was also observed. Mitotic rate was ~ 1per 10 HPF. Metastases were observed in the testicular vein and epididymis of onemouse and in the liver of the other.Conclusion: This report confirms that testicular ultrasound is very useful for thediagnosis of tumors and color doppler can be used to detect areas of angiogenesis intesticular tumors.