Análisis de técnicas de recuperación antigénica para la detección inmunohistoquímica del virus de la diarrea viral bovina

MARINI R; CANAL AM; SALVETTI NR; ORTEGA HH

Invet (Investigación Veterinaria)

Universidad Nacional de Buenos Aires

Lugar: Buenos Aires; Año: 2006 vol. 8 p. 59 - 65

1514-6634

In the present work different methods of antigen recovery were evaluated to allow the immunodetection of the Bovine Viral Diarrhea virus (vDVB) in materials fixed with formol. The effect of the application of proteolytic digestion was analyzed (Ficin, Tripsin, Pepsin, Proteinase K) and its combination with treatment in microwaves using different concentrations and times of incubation for the primary antibody. The most effective treatment was the one carried out with proteinase K, using 1:30 dilution for the primary antibodies, with over night incubation to ambient temperature. We conclude that the formolic fixation affects the antigenicity of the vDVB but these effects can be reverted by specific proteolytic digestion allowing the use of the immunohistochemical routine technique for the diagnosis of the disease.