Characterization of pituitary cell populations in rats with induced polycystic ovaries

FRANCOU M; DURDOS M; SALVETTI NR; BARAVALLE C; REY F; ORTEGA HH

CELLS TISSUES ORGANS

KARGER

Año: 2008 vol. 188 p. 310 - 319

1422-6405

Several hypotheses have been proposed about the pathogenesis of polycystic ovarian syndrome (PCOS); however, the fundamental physiological interactions that initiate the development of follicular cysts have not yet been elucidated. Hence, in this study the proliferation, density, and population of gonadotrophs, mammotrophs, somatotrophs, and corticotrophs of the pituitary glands of rats with induced follicular cysts have been investigated by two experimental models (continuous light exposition and estradiol valerate (EV)-treated rats). Specific immunoreactivity associated with FSH, LH, PRL, GH, and ACTH was evaluated by immunohistochemistry with specific hormone antibodies and proliferation of secretory cells by their colocalization (double-labeling) with PCNA. The results indicate a reduction in the density and proliferation of gonadotrophs in both experimental groups. A reduction in the average density, proliferation, and population of lactotrophs and corticotrophs was also observed in EV-treated animals. However, no significant differences were found in somatotrophs. The present study contributes to the information about alterations of some cell populations that occur in the pituitary gland of rats with PCOS, and will enhance one’s understanding of the pathogenesis this disease.