Growth of lactic acid bacteria of avian origin at low pH and bile

BLAJMAN, J.E.; FUSARI, M.L.; CONTI, G.B.; ROMERO SCHARPEN, A.; BLANCHE, G.P.; ROSSLER, E.; SIGNORINI, M.L.; ROSMINI, M.R.

Tucumán

Simposio; IV Simposio Internacional de Bacterias Lácticas (SIBAL); 2013

CENTRO DE REFERENCIA PARA LACTOBACILOS

A critical aspect of the characterization of probiotic strains is their capacity to avoid biological barriers during digestion. Survival of probiotic bacteria through the gastrointestinal tract is crucial to exert a positive effect when administered in broilers. This study was performed to compare the growth of 11 lactic acid bacteria strains of avian origin and in vitro probiotic properties at low pH and in the presence of bile. A 96 well plate was filled with 150 μl of MRS broth adjusted to pH 2.0 or 150 μl of MRS broth supplemented with 0,6 % p/v ox bile. One point five μl of an overnight grown LAB strain was inoculated. Bacterial optical density (OD), Vmax and Lag time were measured at 630 nm for 24 h every 30 m at 37ºC using a Multi modal micro-plate reader. The experiments were carried out in triplicate for each strain. Statistical analysis was performed using ANOVA followed by Duncan´s test (P