Expression of receptors for TGF-B Superfamily in cows with spontaneous and ACTH-induced cystic ovarian disease

MATILLER V; HEIN GJ; AMWEG AN; RODRÍGUEZ FM; BERTOLI JG; QUERCIA E; REY F; ORTEGA HH

Mar del Plata

Congreso; Reunión anual de SAIC, SAI, SAFE; 2016

Preliminary results shows significant changes in the expression of components of the TGF-B isoformsTGF-B1, 2 and 3, and inhibin / activin / follistatin system in ovarian structures of dairy cattle with spontaneous and ACTH-induced cystic ovarian disease (COD). Given the evidence of its role as an important molecules in paracrine and autocrine signaling pathways that regulate the growth of ovarian follicle,and knowing that this components carry out their actions through binding to two types of membrane receptors, the objective of the present work was examine the expression of TGF-B receptor (TGF-BR) I, TGF-BRII, TGF-BRIII, Activin Receptor (ACVR) IB and ACVRIIB in ovaries of cows with COD previously evaluated for their related ligands. We worked with sections obtained from bovine ovaries with spontaneous COD (n=10), ACTH-inducedCOD (n=10), and controls ovaries (n=10). In 4 um thick sections, the presence and expression of receptors was determined by indirect immunohistochemistry ingranulosa and theca cells. Type I receptors expressed higher staining (p<0.05) in tertiary follicles of spontaneous COD group than controls follicles, and type II receptor ACVRIIB, showed higher immunostaining in theca cells of spontaneous COD group than control. Here we detected changes in the expression of the receptors necessary for the response to the growth factors of TGF-B family in the COD cows ovaries in relation to the controls. The high expression of all receptors analyzed in follicular cysts could be linked to the persistence over time of these structures. We can conclude that alterations found at ligands level materialize a change in signalling favouring these actions at the cellular level. Thus, to unravel intraovarian signaling pathways, we postulate data that may contribute to the knowledge of the pathogenesis of COD and that can be applicable to the design of protocols and therapeutic measures.