Evaluation of enzymes and metabolites associated to lipids and ketone bodies metabolism in dairy cows with follicular persistence

CATTANEO MOREYRA ML; GAREIS NC; ANGELI E; HEIN GJ; ORTEGA HH; SALVETTI NR; RODRÍGUEZ FM; REY F

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias. SAIC, SAB, AAFE, AACyTAL; 2023

Reproduction can be negatively affected due to disruption in lipid andketone body metabolism. Therefore, we evaluate, by immunohistochemistry, theprotein expression of PPARγ receptor, acetyl-CoA carboxylase (ACC, fatty acidsynthesis enzyme), and relevant enzymes of cholesterol metabolism and ketonebodies (3-hydroxy-3-methyl-glutaryl-coenzyme A reductase <HMGR and synthase<HMGS; succinyl-CoA:3-ketoacid CoA transferase <SCOT). In addition, wemeasured the concentrations of β-hydroxybutyrate (BHB), non-esterified fattyacids (NEFA), cholesterol (CHOL) and triglycerides (TG) by using commercialkits. Cows with healthy reproductive tract (n=25) were subjected to estrouscycle synchronization with a G6G-Ovsynch protocol. While cows from the controlgroup (C; n= 5) were only synchronized, those from the persistence groups weretreated with progesterone intravaginally (P4) to induce follicles with 0 (P0;n=5), 5 (P5; n=5), 10 (P10; n=5) and 15 days of persistence (P15; n=5). Ovarieswere obtained by bilateral ovariectomy to finally extract follicular fluid (FF)and ovarian histological sections. A lower expression of ACC and PPARγ wasobserved in theca cells of P15 compared to C (p<0.05). While HMGS expressionwas lower in the granulosa cells of P10 compared to C, P0 and P5 (p<0.05),in theca cells, its expression was lower in the P10 compared to C (p<0.05).In theca cells, the HMGR expression was lower in all the persistence groupscompared to C (p<0.05). Also, the SCOT expression was lower in the granulosacells of P5, P10 and P15 compared to C (p<0.05). In FF, the concentrationsof BHB, CHOL (total, HDL and LDL), TG and NEFA were similar in all the groups(p>0.05). These results suggest a local alteration of the enzymes related tothe lipids and ketone body metabolism in the initial stages of persistence,without evidencing significant changes in their related metabolites in FF.Probably, another extraovarian origin contributes to the ovarianmicroenvironment.