Effects of parenteral supplementation of antioxidant minerals and vitamins in liver NF-ΚB activity and insulin-signaling of dairy cattle during the transition period

ANGELI E; SCHNEIDER A; MATILLER V; REY F; ORTEGA HH; HEIN GJ

Reunión Anual de Sociedades de Biociencias. SAIC, SAB, AAFE, AACyTAL

Congreso; Reunión Anual de Sociedades de Biociencias. SAIC, SAB, AAFE, AACyTAL; 2023

The transition period (last 3 weeks of prepartum until 3 weekspostpartum) is the most critical stage in the lactation of dairy cows,characterized by a proinflammatory state, oxidatve stress and insulinresistance. The aim of this study was to evaluate the effects of parenteralvitamin and mineral supplementation on the hepatic insulin signaling pathway, arelevant proinflammatory factor in the liver, and systemic antioxidant enzymesin cows during the transition period. The supplemented group (SG; n = 11)received injections of 5 ml of the vitamin supplement ADAPTADOR® Vit and 5 mlof the mineral supplement ADAPTADOR® Min (Biogenesis Bagó, Bs. As.; vitamin Apalmitate 3.5% and vitamin E acetate 5%, copper edetate 1%, zinc edetate 4%,manganese edetate 1% and sodium selenite 0.5%) on -60, -30 and 7 days relativeto calving. The control group (CG; n = 11) received two injections of 5 ml of0.9 % sodium chloride solution. Whole blood with heparin was used to measuresuperoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities bycommercial kits, following the manufacturer’s instructions (RANSOD and RANSEL,Randox Laboratories Ltd., Crumlin, UK). The liver protein expression of insulinreceptor (IR), insulin receptor substrate 1 (IRS-1), protein kinase B (alsoknown as Akt), nuclear factor-κB (NF-κB), and their phosphorylated forms wereanalyzed by Western blot. Results showed that the GSH-Px activity was higher inthe SG than in the CG (P<0.05). No differences between groups were observedin SOD activity (P > 0.05). The activation of NF-κB was higher in SG than inCG (P < 0.05). No differences between groups were observed in the insulinsignaling components (P > 0.05). These results suggest that the vitamin andmineral supplementation provided to dairy cows had beneficial effects on theantioxidant system and liver inflammatory state during the transition period.