INVESTIGADORES
MASUELLI Ricardo Williams
artículos
Título:
Caracterización molecular de variedades de vid (Vitis viniferaL.) de calidad enológica por marcadores microsatélites
Autor/es:
MARTÍNEZ, L; CAVAGNARO, P.F.; MASUELLI, R.W.
Revista:
REVISTA DE LA FACULTAD DE CIENCIAS AGRARIAS DE LA UNIVERSIDAD NACIONAL DE CUYO
Editorial:
UNIV NACIONAL CUYO
Referencias:
Año: 2006 p. 77 - 86
ISSN:
0370-4661
Resumen:
SSR markers characterized seven grapevine
varieties used for high quality wines in Argentina.
Six of the 8 primers used gave reproducible
band profiles. The number of alleles
per locus varied from 5 to 10, with a total of 42
and 1 to 7 unique alleles. Three to 7 SSR
genotypes per locus were found, with a total of
28. Tempranillo and Chenin showed 6 and 5
unique alleles, respectively, taking in account
all the primers assessed. The observed
heterozygosity varied between 42.9 and 100 %,
while the expected heterozygosity ranged from
64.3 to 87.8 %. VrZAG79 was the most
informative locus (PIC 84.9 % and ne 8.17),
while VrZAG62 was the least informative.
Combining the use of 6 primers, the accumulative
probability of missidentifying two random
different genotypes as the same, was very low:
7.68 x 10-05. The UPGMA cluster analysis
separated the French varieties (Merlot, Pinot
Noir, Cabernet Sauvignon), and Syrah from
Tempranillo and Bonarda. This technique can
provide a service to grapevine nurseries and
farmers interested in certifying the genetic
identity of the materials commercialized by them.e 8.17),
while VrZAG62 was the least informative.
Combining the use of 6 primers, the accumulative
probability of missidentifying two random
different genotypes as the same, was very low:
7.68 x 10-05. The UPGMA cluster analysis
separated the French varieties (Merlot, Pinot
Noir, Cabernet Sauvignon), and Syrah from
Tempranillo and Bonarda. This technique can
provide a service to grapevine nurseries and
farmers interested in certifying the genetic
identity of the materials commercialized by them.-05. The UPGMA cluster analysis
separated the French varieties (Merlot, Pinot
Noir, Cabernet Sauvignon), and Syrah from
Tempranillo and Bonarda. This technique can
provide a service to grapevine nurseries and
farmers interested in certifying the genetic
identity of the materials commercialized by them.