Application of residual cooking oil to improve the production of a low-toxic biosurfactants extract

HAIDAR, CARLA NAHIR; MALIZIA, FLORENCIA; MENACHO MÁRQUEZ, MAURICIO; NERLI, BIBIANA BEATRIZ; PELLEGRINI MALPIEDI, LUCIANA

Bioresource Technology Reports

Elsevier

Lugar: Amsterdan; Año: 2022

2589-014X

Residual cooking oil (RCO) bioconversion into value-added products is one of the bestoptions to manage this waste. Thus, the feasibility of producing lipopeptides byPseudomonas syringae pv tabaci using this residue is reported for the first time. Surfaceactivity, stability, and cytotoxicity of biosurfactants were explored by surface tension,emulsification indexes (EI24), and mammalian cell viability assays. It was demonstratedthat a mixture of RCO and glycerol (1:1) as carbon source increased the production titerfrom 1.47 to 2.70 g/L, syringopeptin being the main component. The produced lipopeptideswere able to reduce the water surface tension down to38 mN/m and emulsify effectively (EIup to 67%) a wide variety of hydrophobic compounds at 0.10 g/L. Moreover, theselipopeptides at 1.0 g/L resulted to be highly compatible with mammalian cells (viabilitysuperior to 70%), demonstrating that at the working concentrations are non-toxic and thus,adequate for food and pharmaceutical applications.