New acrylic bone cements conjugated to vitamin E: curing parameters, properties and biocompatibility

MENDEZ,J.A.; AGUILAR,M.R.; ABRAHAM,G.A.; VAZQUEZ,B.; DALBY,M.; DI SILVIO,L.; SAN ROMAN,J

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH

John Wiley & Sons, Inc.

Lugar: New York, USA; Año: 2002 vol. 62 p. 299 - 307

0021-9304

Acrylic bone cement formulations with anti-oxidant character were prepared by incorporation of a methacrylic monomer derived from vitamin E (MVE). Increasing concentrations of this monomer provided decreasing peak temperature values, ranging from 62 to 36ºC, and increasing setting time with values between 17-25 min. Mechanical properties were evaluated by compressive and tensile tests. Compressive strength of the new formulations were superior to 70 MPa in all cases and Young’s modulus showed a significant increase for the cements prepared with 15-25 wt-% MVE. However, the cement containing 25 wt-% MVE showed a significant decrease in tensile properties. Biocompatibility of the new formulations was studied in vitro. The analysis of the effect of leachables from cements into the media showed continued cell proliferation and cell viability with a significant increase for the cement containing 15 wt-% MVE. This formulation also showed a significant increase in cellular proliferation over a period of 7 days for the Alamar Blue test. The cells were able to differentiate and express phenotypical markers in presence of all materials. A significant increase in alkaline phosphatase activity being observed on the cements prepared in presence of 15-25 wt-%MVE compared to PMMA. Morphological assessment showed that the human osteoblast (HOB) cells were able to adhere and proliferate on all the cements.