Topographical and compositional effects of biomaterials during in situ differentiation of human mesenchymal stem cells

ALDANA, ANA AGUSTINA; RIVERO GUADALUPE; FRONTINI LOPEZ, Y. R.; LIVERANI, LILIANA; UHART, M. ; BUSTOS, D. M.; ABRAHAM, G. A.; BOCCACINI, A. R.

Berlin

Simposio; IX Reunión Anual Red de Científicos Argentinos en Alemania; 2019

It is known that Human adipose-derived mesenchymal stem cells (hASCs) accelerate the osteointegration of bone grafts and improve the efficiency in the formation of uniform bone tissue, providing a practical and clinically attractive approach in bone tissue regeneration [1]. In our collaboration projects, the morphological and biological cues were modulated to influence hASCs behavior. On one handthe effect of nanofibrous biomimetic matrices composed of poly(ε-caprolactone) (PCL), hydroxyapatite nanoparticles(nHA)and 14-3-3 protein on the initial stages of hASCs osteogenic differentiationwas explored [2]. On the other hand, gelatin methacrylate (GelMA) / alginate / 14-3-3 protein hydrogel scaffolds were fabricated by 3D bioprinting.In both projects, cells were characterized by flow cytometry and induction to differentiationto adipogenic and osteogenic lineages. The isolated hASCs were induced to differentiate to osteoblasts over all scaffolds,and viability of the hASCs were found to be similar. However, the activity of alkaline phosphatase (ALP) asearly osteogenic marker in the protein-incorporated scaffolds was three or more times higher than the measured in scaffolds without. The cell adhesion was similar in nanofibrous scaffolds but it was enhanced in bioprinted hydrogels by adding 14-3-3 protein.The results are relevant for future developments of both scaffolds for bone tissue engineering given the positive effect of 14-3-3 protein on both cell proliferation and osteogenecity.