Reactive oxygen species and nitric oxide are involved in polyamine-induced growth inhibition in wheat plants

RECALDE L.; VÁZQUEZ A.; GROPPA M.D.; BENAVIDES M.P.

PROTOPLASMA

SPRINGER WIEN

Lugar: Viena; Año: 2018 vol. 255

0033-183X

Polyamines (PAs) produce H O and nitric oxide (NO) during their normalcatabolism and modulate plant growth and development. To explore thebiochemical basis of PAs-induced growth inhibition in Triticum aestivum Lseedlings, we examined the role of O , H O or NO in shoot and rootdevelopment. Although all PA treatments resulted in a variable reduction of rootand shoot elongation, spermine (Spm) caused the greater inhibition in a similarway to that observed with the NO donor, sodium nitroprusside (SNP). In bothcases, O production was completely blocked whereas H O formation was highin the root apex under SNP or Spm treatments. Catalase recovered root and shootgrowth in SNP but not in Spm-treated plants, revealing the involvement of H Oin SNP-root length reduction. The addition of the NO scavenger, cPTIO, restoredroot length in SNP- or Spm-treated plants, respectively, and partially recoveredO levels, compared to the plants exposed to PAs or SNP without cPTIO. Astrong correlation was observed between root growth restoration and Oaccumulation after treating roots with SNP + aminoguanidine, a diamine oxidaseinhibitor, and with SNP + 1,8-diaminoctane, a polyamine oxidase inhibitor,confirming the essential role of O formation for root growth and theimportance of the origin and level of H O . The differential modulation of wheatgrowth by PAs through reactive oxygen species or NO is discussed.