Does Vitamin D Insufficiency Influence Prebiotic Effect on Calcium Absorption and Bone Retention?

SEIJO, MARIANA; BONANNO, MARINA N.; BRYK, GABRIEL; ZENI CORONEL, MAGALI E.; PITA MARTIN DE PORTELA, MARIA LUZ; ZENI, SUSANA N.

Calcified Tissue International

Springer

Año: 2022

Higher calcium (Ca) absorption would partially compensate for Ca intake below requirements for bone health. Previously,we found that GOS/FOS prebiotic mixture (PM) increases Ca absorption in the colon and retention in bone. Ca absorptionand retention are regulated by vitamin D (VD). Hence, it is relevant to explore whether VD insufciency infuences theefect of the PM in the colon. The efect of the PM on Ca, phosphate (IP), and magnesium (Mg) absorption and retentionunder conditions of VD sufciency and insufciency (VDInsuf) was compared using a preclinical model of VDInsuf andlow bone mass. Ovariectomized rats were fed isocaloric semisynthetic diets according to AIN-93 M. The diets varied in Ca(0.5% or 0.3%), VD [100 IU% (+D) or 0 IU% (−D)], and PM (2.5% or 0%) content. The following eight groups were studied:+D0.5;+D0.3;+DPM0.5;+DPM0.3;−D0.5;−D0.3;−DPM0.5; and−DPM0.3. Irrespective of Ca content, VDInsufdid not afect the prebiotic efect of the PM on caecum pH, lactobacillus colony growth, or Mg absorption but signifcantlydecreased its efect on colonic crypt length and cell/crypt and Ca and IP absorption. The PM failed to counterbalance thepro-infammatory efect of VDInsuf. Moreover, bone retention i.e., bone mineral content and density, bone volume, andbone quality parameters were signifcantly lower (p