INVESTIGADORES
ISLA Maria Ines
artículos
Título:
Antimicrobial activity of selected plant species from “the Argentine Puna” against sensitive and multi-resistant bacteria
Autor/es:
ZAMPINI I.C., CUELLO S., ALBERTO M.R., ORDOÑEZ R.M., D’ ALMEIDA R., SOLORZANO E.; ISLA, M. I
Revista:
JOURNAL OF ETHNOPHARMACOLOGY
Editorial:
ELSEVIER
Referencias:
Año: 2009 vol. 124 p. 499 - 505
ISSN:
0378-8741
Resumen:
Aim: The aim of this study was to evaluate the group of selected plants for antimicrobial properties against a panel of sensitive and multi-resistant Gram (+) and Gram (-) bacteria. Materials and methods: The antimicrobial activity of tinctures and aqueous extracts  (Baccharis boliviensis, Chiliotrichiopsis keidelii; Chuquiraga atacamensis; Fabiana bryoides; Fabiana densa; Fabiana punensis; Frankenia triandra; Parastrephia lucida; Parastrephia lepidophylla; Parastrephia phyliciformis; Tetraglochin cristatum) was determined using the agar macrodilution and broth microdilution methods recommended by the Clinical and Laboratory Standards Institute (CLSI, formerly NCCLS). The antibiotic resistant clinical strains were isolated from nosocomial infection in human lesions of skin and soft parts. Results: The ethanolic extracts of 11 plant species inhibited the growth of one or more of the following strains: Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterobacter cloacae, Morganella morganii, Pseudomonas aeruginosa. Ethanol extracts (tinctures) of aerial parts of Baccharis, Fabiana and Parastrephia showed the highest levels of antibacterial activity on methicillin, oxacillin and gentamicin resistant Staphylococcus with MIC values from 20 to 150 μg/ml. Baccharis boliviensis and Fabiana bryoides were more active than the other plant species on Enterococcus faecalis with different phenotype. The most interesting activity on multiresistant Gram negative strains was obtained from Chuquiraga atacamensis. Parastrephia species showed activity against E. cloacae, P. aeruginosa and P. mirabilis. The ethanolic extracts exhibited stronger activity and broader spectrum of action than aqueous extracts. The extracts were bactericidal in most cases. Conclusions: The presence of antibacterial activity in Puna plant extracts against multiresistant bacteria give support to their traditional use for treating conditions associated with microorganisms in humans and animals and consequently seems promising for the treatment of multiresistant bacteria.