INVESTIGADORES
ISLA Maria Ines
artículos
Título:
Free radical scavenging activity,antimutagenicity and non genotoxicity of antimicrobial proteins isolates from Cyphomandra betacea fruits and Solanum tuberosum tubers.
Autor/es:
ROXANA MABEL ORDÓÑEZ , IRIS CATIANA ZAMPINI, FABIOLA RODRÍGUEZ, FLORENCIA CATTANEO, JORGE ESTEBAN SAYAGO, MARÍA INÉS ISLA
Revista:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Editorial:
AMER CHEMICAL SOC
Referencias:
Año: 2011 vol. 59 p. 8655 - 8660
ISSN:
0021-8561
Resumen:
In this study, antioxidant activities in free-radical-mediated oxidative systems and the genotoxic/antigenotoxic effects
of two proteins with molecular mass around 17 kDa, purified from Solanum betaceum fruits (cyphomine) and Solanum tuberosum
tubers (solamarine), were investigated. Both proteins inhibited uric acid formation with IC50 values between 55 and 60 μg/mL, and
both proteins were able to reduce oxidative damage by scavenging hydroxyl radicals and superoxide anion in a dose-dependent
manner. Furthermore, the DPPH? reduction assay showed SC50 values of 5573 μg/mL. Cyphomine and solamarine were able to
retain their antioxidant activity after heat treatment at 80 C for 15 min. Allium cepa and Salmonella/microsome assays showed no
genotoxic and mutagenic effects. Solamarine showed an antimutagenic effect against a direct mutagen (4-nitro-o-phenylenediamine).
Consequently, the present study showed that the investigated proteins are promising ingredients for the development of
functional foods with a beneficial impact on human health and an important source for the production of bioactive
peptides.