ISLA Maria Ines
Antimicrobial phenylpropanoids from the Argentinean highland plant Parastrephia lucida (Meyen) Cabrera
ROMINA E. DALMEIDA A, MARIA R. ALBERTO, CRISTINA QUISPE, GUILLERMO SCHMEDA-HIRSCHMANN, MARÍA I. ISLA
JOURNAL OF ETHNOPHARMACOLOGY
ELSEVIER IRELAND LTD
Lugar: Amsterdam; Año: 2012 vol. 142 p. 407 - 414
Ethnopharmacological relevance: The Argentinean highland plant Parastrephia lucida (Meyen) Cabrera is used in traditional medicine as an antiseptic and anti-inflammatory crude drug.Aim of the study: To relate the antimicrobial effect of the crude drug with the constituents of the active fractions and traditional use.Materials and methods: Assay-guided isolation of the methanol (MeOH) plant extract was carried out using bacteria and yeasts as target organisms. Both ATCC and local strains were included in the study.The antimicrobial fractions and compounds were detected by bioautographic assays. Minimuminhibitory concentrations (MIC) of each extract and fraction were determined and compared withreference antibiotics. Fractions were analyzed by HPLC?DAD, GC?MS, 1H NMR and 13C NMR.Results: From the MeOH extract of the plant, assay-guided isolation of the antimicrobial constituents led to 12 phenylpropanoids and two simple phenolics. Most of the compounds occurring in the active fractions were E-caffeoyl or E-cinnamoyl esters including prenyl and phenethyl derivatives. The MIC values of the most active fractions ranged between 12.5 and 200 mg/mL against reference strains and local isolates of Staphylococcus aureus and Enterococcus faecalis.Conclusions: The antimicrobial effect found in the crude drug was associated with mixtures ofphenylpropanoids, including prenyl and phenethyl esters of caffeic and cinnamic acids. The resultssupport at least in part the traditional use of the plant as local antiseptic.