Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products

MICAELA BAGLIONI; ALEXANDER FRIES; JAN-MATHIS MÜLLER; ALEJANDRA OMARINI; MICHAEL MÜLLER; JAVIER D. BRECCIA; LAURA S. MAZZAFERRO

Applied Microbiology and Biotechnology

Springer Science and Business Media Deutschland GmbH

Año: 2024 vol. 108

The fungal diglycosidase α-rhamnosyl-β-glucosidase I (αRβG I) from Acremonium sp. DSM 24697 catalyzes the glycosyla- tion of various OH-acceptors using the citrus flavanone hesperidin. We successfully applied a one-pot biocatalysis process to synthesize 4-methylumbellipheryl rutinoside (4-MUR) and glyceryl rutinoside using a citrus peel residue as sugar donor. This residue, which contained 3.5 % [w/w] hesperidin, is the remaining of citrus processing after producing orange juice, essential oil, and peel-juice. The low-cost compound glycerol was utilized in the synthesis of glyceryl rutinoside. We imple- mented a simple method for the obtention of glyceryl rutinoside with 99 % yield, and its purification involving activated charcoal, which also facilitated the recovery of the by-product hesperetin through liquid-liquid extraction. This process presents a promising alternative for biorefinery operations, highlighting the valuable role of αRβG I in valorizing glycerol and agricultural by-products.