INVESTIGADORES
ROSSI franco Ruben
congresos y reuniones científicas
Título:
FUNTIONAL REDUNDANCY OF Arabidopsis thaliana ADC ISOFORMS IN RESPONSE TO Botrytis cinerea INFECTION
Autor/es:
FRANCO RUBÉN ROSSI; MARÍA MARINA; OSCAR ADOLFO RUIZ; FERNANDO LUIS PIECKENSTAIN
Lugar:
Mendoza
Reunión:
Congreso; Reunion Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2012
Resumen:
Arginine
decarboxylase (ADC) catalyzes the first step of polyamine (PA) biosynthesis. The
model plant Arabidopsis thaliana
harbors two ADC isoforms (AtADC1 and AtADC2), which are differentially
regulated by different stresses, but no information about their regulation
under biotic stress is available. This study aimed to determine the role AtADCs in defense against infection by Botrytis
cinerea. The effect of infection on the expression of each isoform,
PA levels and lesion size were evaluated in null adc1 and adc2 mutants, as
compared to wild-type (WT, Col-0)
plants.
Infection increased the expression
of both isoforms in WT plants, as compared to non-inoculated controls. AtADC2 expression was increased in infected
adc1 mutants, as compared to
non-inoculated adc1 plants.
Reciprocally, infection enhanced AtADC1
expression in adc2 mutants, as
compared to non-inoculated adc2
plants. WT plants and both adc
mutants exhibited increased Put levels in response to infection, as well as similar
levels of tissue damage.
Results demonstrate
that B. cinerea infection induces
AtADC expression, but none of the isoforms seems to be defense-specific, since
each one was induced by infection when the other was mutated. This functional
redundancy, as well as the previously reported lethal phenotype of double (adc1-adc2) mutants render difficult to
assess the contribution of ADC to resistance.